Nuclear deoxyribonucleic acid-dependent ribonucleic acid polymerases from Saccharomyces cerevisiae.
نویسندگان
چکیده
Two deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerases (I, II) have been solubilized from isolated Saccharomyces cerevisiae nuclei. The enzymes can be separated by chromatography on O-diethylaminoethyl Sephadex. Both enzymes are active with high-molecular-weight nuclear yeast DNA, although RNA polymerase I has a higher affinity for polydeoxy-adenylic-thymidylic acid and RNA polymerase II for denatured DNA. RNA polymerase I is active only with manganese. alpha-Amanitin inhibits only the activity of RNA polymerase II.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 114 1 شماره
صفحات -
تاریخ انتشار 1973